ELISA Reagents

Surmodics™ IVD’s ELISA Reagents

The enzyme-linked immunosorbent assay or ELISA is one of the most commonly used analytical biochemistry assays. Through their reliability and consistency, ELISA’s have come to play a major role in detecting disease. Surmodics™ IVD provides assay developers the ELISA reagents they need to build robust, accurate and reproducible ELISA’s. Our extensive portfolio of ELISA reagents helps to increase the sensitivity, specificity and stability of ELISA’s. In addition, each of our ELISA reagents were designed to help tackle difficult technical issues such as non-specific binding, matrix interferences and more; in turn, our ELISA reagents help assay developers in the continued optimization of their ELISA technology.

Visit the link below to learn more about Surmodics IVD’s ELISA reagents.

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Explore Surmodics IVD’s ELISA Reagents

ELISA reagents are widely used as a method of rapidly testing and quantifying antibodies against bacteria, viruses, as well as other material antigens. In the face of regional epidemics and global pandemics, ELISA is being increasingly used to aid in the detection of antigen (infectious agent) or antibody. This is largely due to the general sensitivity and simplicity of ELISA’s overall. This makes it a useful tool in the detection of various antigens and antibodies, such as hormones, viruses, and other toxins.

Ensuring you have the right tools to build consistent and reliable ELISA kits is essential and that’s where Surmodics IVD comes in. For over 40 years, we have provided assay manufacturing companies and researches trusted ELISA reagents.

Surmodics IVD’s ELISA reagents include the industry’s gold standard in protein stabilizers/blocking buffers designed to increase sensitivity and specificity while decreasing non-specific binding. We also offer best-in-class detection substrates for color (TMB, ABTS, PNPS, BCIB/NBT) and light (Chemiluminescent) development as well as a selection of accessory reagents including our high-performance diluents designed to reduce matrix interferences including Human Anti-Mouse Antibodies (HAMA) and Rheumatoid Factor (RF) interferences.

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We also manufacture and supply a line of microarray slides and surface technology as well as supply high quality antigens and antibodies from our partner company, DIARECT™ located in Germany.

Surmodics™ IVD is also your “one stop shop” for building immunoassays. Our immunoassay reagents are designed to increase the sensitivity, specificity and stability of immunoassays, including ELISA’s, point-of-care devices and more diagnostic applications. These immunoassay reagents are critical for the accuracy and reliability of immunoassays.

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Dried Protein Stabilizers and Blockers (Immunoassay Stabilizers):

Unmatched stabilization of dried proteins and blocking efficacy in a one-step process to streamline manufacturing of immunoassays including ELISA’s.

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Sample/Assay Diluents:

Both MatrixGuard™ Diluent and Surmodics Assay Diluent (Protein-Free) provide the gold standard in reducing false positives in your assay.

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In-Solution Protein Stabilizers (Conjugate Stabilizers):

The gold standard for stability of protein conjugates at working strength concentration for ELISA/EIA, ELISpot, RIA and immunoblot/western blot applications.

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ELISA Substrates

Surmodics IVD’s portfolio of BioFX™ colorimetric and chemiluminescent substrates offers the stability, low background and sensitivity needed to meet the demands of assay manufacturers.

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Stop Solutions and Accessory Reagents

Surmodics IVD offers several dry-blend and liquid stop solutions and accessory reagents including ELISA plate coating solutions and ELISA wash buffers.

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DIARECT Antigens and Antibodies

As the exclusive North American representative of DIARECT products, Surmodics IVD offers a comprehensive panel of autoimmune disease, infectious disease, allergy antigens and antibodies that provide outstanding assay performance for in vitro diagnostics applications.

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Surmodics IVD provides ELISA reagents that are distinctly differentiated due to their high-performance formulations, which make for a quicker path to commercialization. Whether you’re in search of a diluent, protein stabilizer, an ELISA substrate, or any related products, Surmodics IVD offers many products and solutions to meet your needs.

All Surmodics IVD’s ELISA reagents and additional products are manufactured in our ISO 13485:2016 and 9001:2015 certified facility. Expect lot-to-lot consistency, reproducibility, and optimal performance from products manufactured by Surmodics IVD.

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Which reagents are used in an ELISA test?

There are several reagents that are typically used in an ELISA. The standard ELISA reagents used include a detection antigen or antibody, ELISA coating buffer, washing buffer, dried protein stabilizers/blockers, sample or assay diluents should the sample needed to be treated, in-solution stabilizers or conjugate stabilizers, a detection substrate and a stop solution. Surmodics IVD offers each type of reagent needed to build an ELISA.

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What is an ELISA test used for?

ELISA tests are used in order to detect and measure the antibodies present in the bloodstream. Particularly, the ELISA test is used to measure whether a patient's blood possesses certain antibodies in relation to certain infectious diseases. These tests can be used as a means of diagnosing various viruses and diseases.

What are the steps of ELISA?

In terms of the steps of ELISA testing, they also vary depending on the nature of the test. However, an example of Sandwich ELISA is as follows:

  1. Prepare the surface
  2. Block nonspecific binding sites on the surface
  3. The sample is then applied to the site to be captured by the antibody
  4. The plate is then washed thoroughly to remove the unbound antigen
  5. Next, the specific antibody needed to conduct the analysis is added, and the antibody binds to the antigen
  6. Enzyme-linked secondary antibodies are then applied to detect antibodies
  7. A chemical is added that is converted by the enzyme
  8. Researchers then measure the absorbance of electrochemical or fluorescence to determine the quality and overall presence of the antigen