Surmodics IVD Library

ELISA Assay Solutions & Protocol

Enzyme-linked immunosorbent assays (ELISA’s) are plate-based enzyme assays used for identifying and quantifying sample proteins, peptides, antigens, and antibodies that are targets in research of a specific compound. The identification of a specifically targeted protein in an ELISA array is completed by using antibodies to immobilize the protein and detect the presence of the protein targeted.

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ELISA Blocking Reagents

Surmodics™ IVD has a wide selection of reliable, ready-to-use ELISA blocking reagents for assay manufacturing companies and laboratory professionals looking to build sensitive, reproducible immunoassays. We offer several blocking reagents for immunoassays, including point-of-care applications, ELISA’s and more.

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How Do Immunoassay Tests Work

Immunoassay Tests are biochemical tests that detect disease and works on the principle of antigen-antibody reaction. It aims at measuring the presence and the concentration of an analyte. Researchers and health care professionals utilize immunoassays to detect different kinds of antibodies.

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Guide to Determining Protein Stability

Proteins play the most critical role in immunoassays. They act as the detection antigen or antibody that assay developers use to determine whether a disease state is present. Preserving these proteins is essential in determining accurate results.

Read our Guide to Determining Protein Stability

ELISA Wash Buffers

ELISA wash buffers were developed as a high performing washing solution to be used in a variety of versatile ELISA formats. Surmodics™ IVD’s BioFX™ Tris Buffered Saline (TBS) Wash Solution‐10X Concentrate contains non‐ionic surfactant, which does not interfere with assay reactants and reduces non‐specific binding.

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