Surmodics IVD Library

ELISA Assay Solutions & Protocol

Enzyme-linked immunosorbent assays (ELISA’s) are plate-based enzyme assays used for identifying and quantifying sample proteins, peptides, antigens, and antibodies that are targets in research of a specific compound. The identification of a specifically targeted protein in an ELISA array is completed by using antibodies to immobilize the protein and detect the presence of the protein targeted.

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ELISA Blocking Reagents

Surmodics™ IVD has a wide selection of reliable, ready-to-use ELISA blocking reagents for assay manufacturing companies and laboratory professionals looking to build sensitive, reproducible immunoassays. We offer several blocking reagents for immunoassays, including point-of-care applications, ELISA’s and more.

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How Do Immunoassay Tests Work

Immunoassay Tests are biochemical tests that detect disease and works on the principle of antigen-antibody reaction. It aims at measuring the presence and the concentration of an analyte. Researchers and health care professionals utilize immunoassays to detect different kinds of antibodies.

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Guide to Determining Protein Stability

Proteins play the most critical role in immunoassays. They act as the detection antigen or antibody that assay developers use to determine whether a disease state is present. Preserving these proteins is essential in determining accurate results.

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ELISA Wash Buffers

ELISA wash buffers were developed as a high performing washing solution to be used in a variety of versatile ELISA formats. Surmodics™ IVD’s BioFX™ Tris Buffered Saline (TBS) Wash Solution‐10X Concentrate contains non‐ionic surfactant, which does not interfere with assay reactants and reduces non‐specific binding.

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Elisa Kits

ELISA kits should be sensitive, specific and stable. Surmodics IVD’s immunoassay components for ELISA kits are a reliable resource in the diagnostic industry. Surmodics IVD proudly offers several immunoassay components for both competitive ELISA formats and sandwich ELISA formats.

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The standard components in an ELISA kit include antibodies, antigens, dried protein stabilizers and blockers, wash buffers, in-solution protein stabilizers, substrates, stop solutions and sample/assay diluents if needed. Surmodics IVD acts as a one-stop-shop for assay developers to purchase individual components of an ELISA when building ELISA kits. Our extensive portfolio of ELISA products helps overcome technical issues and help assay developers in the continued optimization of their ELISA technology.

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Non-specifc Binding: What You Need to Know

Non-Specific binding (NSB) refers to an occurrence of an antibody binding to unintended proteins, receptors, or transporters. This binding of assay antibodies is not correlated with the specificity of the antibodies.

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Difference Between a Diluent and a Solvent: Definitions and More

To dilute something is to reduce its potency, viscosity, or concentration. The definition of a diluent in immunochemistry is a liquid solution that reduces the potency of another substance or liquid. In immunoassays, using a sample or assay diluent can help reduce false positives and block matrix interferences while maintaining the assay’s clinical utility.

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