Microarray Slides/Surfaces

molecular diagnostics

Surmodics TRIDIA™ surface coatings optimize DNA, RNA, protein, and cell attachment for molecular diagnostic and immunoassay applications, reducing non-specific background and improving sensitivity. These versatile coatings bind molecules to a variety of surfaces and geometries and may be customized for selectivity using passivating polymers and reactive groups.

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Dna oligonucleotide binding to microarray slide surfaces

TRIDIA™ NHS and TRIDIA™ HD surfaces have superior binding compared to other microarray surfaces. They bind amine-modified oligos, DNA or RNA.

Common applications: DNA arrays, SNP analysis and array CGH

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Biotinylated oligonucleotide was attached either through a 5’amine group or hydroxyl group depending on the surface tested. Streptavidin-Cy5 was used for detection and quantification of oligo bound to surface.

TRIDIA™ and CodeLink® slides and coatings immobilize biological entities to surface materials.

  • Reactive chemistry is homogeneous across entire surface for high signal and consistent results
  • Passivating polymer increases sensitivity by preventing non-specific binding of unwanted biomolecules
  • Thin, versatile photochemistry allows coating of various geometries and surface materials

MICROARRAY SLIDES/SURFACE CHEMISTRY

Analysis of Troponin on a Protein Microarray

Lower backgrounds and better sensitivity with TRIDIA™ EP and TRIDIA™ BA Surfaces. The TRIDIA™ slide displays more distinct spots with consistently low background across the slide.

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Troponin I sandwich immunoassay. Capture antibody–mouse anti-troponin I (AbCam); primary detection antibody-rabbit anti-troponin antibody; secondary detection antibody-goat anti-rabbit-Cy3. Scanned on Axon 4200 AL scanner in the 632 nm channel.