Immunoassay Reagents

Surmodics™ IVD’s Immunoassay Reagents:

Surmodics™ IVD is your “one stop shop” for building immunoassays. Our immunoassay reagents are designed to increase the sensitivity, specificity and stability of immunoassays, including ELISA’s, point-of-care devices and more diagnostic applications. These immunoassay reagents are critical for the accuracy and reliability of immunoassays.

Surmodics IVD’s immunoassay reagents are the gold standard in product performance and offer assay developers a complete set of tools for building consistent and reliable immunoassays.

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Explore Immunoassay Reagents

If you are looking for which immunoassay reagent is best suited for your immunoassay, it can be helpful to understand what technical issue you want to overcome and/or how you wish to optimize your immunoassay. Surmodics IVD’s dried protein stabilizer and blocker immunoassay reagents help to stabilize the antigen or antibody to the surface over time while blocking interferences that may be impacting the accuracy of the immunoassay. Additionally, our sample or assay diluents effectively eliminate interferences from discordant samples and block heterophiles such as HAMA, RF from interfering with an immunoassay. In addition, Surmodics IVD’s TMB substrates offer best-in-class stability, sensitivity and lot-to-lot consistency and help assay developers achieve their optimal level of detection. We also offer a line of accessory reagents including stop solutions, which offers assay developers a safer alternative to use at this step. Surmodics IVD is also the exclusive representative of DIARECT™ antigens and antibodies in North America. DIARECT’s antigens and antibodies have a proven track record for quality and sensitivity in ELISA, Western Blot, bead-based applications and microarrays.

Each of these families of immunoassay reagents were designed to optimize immunoassays and help tackle technical issues.

Contact us today to request free samples and connect with our R&D Team for further support in optimizing your immunoassay.

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DIARECT™ Antigens & Antibodies:

Surmodics IVD’s antigens and antibody immunoassay reagents have a track record of innovation within the autoimmune, infectious disease and allergen product lines and are supported by large-scale production capabilities necessary for quality immunoassay development. A deep product portfolio, lot-to-lot consistency and large batch sizes provide in vitro diagnostic manufacturers faster time to market.

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ELISA Protein Stabilizers, Diluents & Blockers:

Surmodics IVD’s protein stabilizers, diluents and blocker immunoassay reagents offer assay developers a complete set of tools for stabilizing proteins over time while reducing interferences.

Surmodics IVD’s dried protein stabilizers and blockers or immunoassay stabilizers, preserve the conformation and activity of dried proteins coated on a wide range of surfaces, keeping antibodies and antigens at peak performance for long durations. At the same time, the blocking mechanisms in these reagents reduce non-specific binding of interfering proteins to maximize assay sensitivity. Our immunoassay stabilizers are the industry gold standard for stability and blocking efficacy. The result is immediate improvement of assay performance in a one-step process for streamlined manufacturing.

Visit the link below to learn more about our dried protein stabilizers and blocker immunoassay reagents.

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Surmodics IVD’s sample or assay diluent immunoassay reagents offer assay developers unsurpassed performance in a 1-component diluent. Due to the variability within patient samples, different blocking strategies are needed to dilute samples to achieve maximum assay performance. Both MatrixGuard™ and Surmodics IVD’s Assay Diluent (Protein-Free) provide the gold standard in reducing false positives in your assay. MatrixGuard (protein) and Assay Diluent (protein-free) formulations provide two options to use when different methods are needed to block matrix interferences, while maintaining the clinical utility of the assay.

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Surmodics IVD’s protein and conjugate stabilizer immunoassay reagents offer assay developers the gold standard for stability of protein conjugates at working strength concentrations. Assay developers require in-solution protein stability for immunoassay applications. Conjugate stabilizers preserve activity of the enzyme (HRP or AP) and the antibody or antigen of the conjugate complex. This allows for the storage of conjugated proteins at lower use concentrations to facilitate optimization during assay development and manufacturing.

Surmodics IVD’s StabilZyme™ family provides unparalleled long-term stability of protein conjugates at working strength concentrations. Our formulations for HRP conjugates, AP conjugates and general protein applications ensure that assay developers have a complete set of tools to address the labile nature of each protein of interest. These reagents immediately improve assay performance and manufacturability of assays by maintaining protein activity and longer shelf life.

Visit the link below to learn more about our protein and conjugate stabilizer immunoassay reagents.

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BioFX™ Substrates:

To produce the most robust possible assays, developers need to evaluate many components, including antibody/antigen systems, blocking and stabilization, and enzyme detection substrates. While the choice of antibody or antigen is often the primary consideration, the choice of substrate also has a major effect on performance. The Surmodics IVD TMB microwell substrate family outperforms competitors for low background, stability and sensitivity with a variety of kinetic rate substrates. Our one-component formulations provide optimal signal generation, longer shelf lives and the ability to streamline manufacturing of assays.

Surmodics IVD offers both HRP and AP formulations of membrane substrates to provide a “one stop shop” for a variety of applications including the development of point-of-care technologies, dot blots, western blots, ELISpots and more. BioFX™ Membrane Substrates offer unparalleled consistency and quality and are manufactured under stringent conditions that minimize variation among production lots.

Visit the link below to learn more about our TMB substrate immunoassay reagents.

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Stop Solutions and Support Reagents:

Surmodics IVD also offers a line of ELISA stop solutions and accessory immunoassay reagents. Surmodics IVD offers both AP and TMB stop solutions as well as immunoassay reagents for Along ELISA plate coating, immunohistochemistry and a wash solution. Along with all of Surmodics IVD’s reagents, our ELISA stop solutions and accessory reagents are manufactured in our ISO 13485:2016 and 9001:2015 certified facility allowing us to offer assay developers consistency and the highest level of quality.

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Frequently Asked Questions:

What is an immunoassay?

Immunoassays measure analyte concentration via responses that are associated with antibody and antigens. Immunoassays are common procedures for measuring analytes usually occurring at below average concentrations that are unable to be determined precisely by other less expensive methods and procedures. Common uses of immunoassay methods include measurement of hormones, drugs, tumor markers, specific proteins and markers of cardiac trauma or injury.

How is an immunoassay performed?

Immunoassays operate in a variety of ways. They search high and low for molecules that are called "analytes." These molecules often are classified as being proteins. Learn more about how immunoassay tests work

What are the types of immunoassays?

There are five types of immunoassays that are generally used:

Enzyme Immunoassay (EIA):

One of the most commonly used types of immunoassays is EIA, which uses enzymes to label either the antibody or antigen. The most commonly used EIA methods for identification of infectious diseases is the enzyme-linked immunosorbent assay (ELISA).

Radioimmunoassay (RIA):

Radioimmunoassays determine antibody levels by using radioisotopes. An antigen is introduced to the radioisotope and the subsequent radioactivity of the antibody component is measured. With RIA, laboratory professionals can use just a single drop of blood to determine and measure the concentration of biochemical substances.

Fluoroimmunoassay (FIA):

FIAs are a type of immunoassays that utilize a fluorescent label or an enzyme label, which will then act on the substrate to form a fluorescent product. Fluorescent measurements are inherently more sensitive than colorimetric (spectrophotometric) measurements. Therefore, FIA methods have greater analytical sensitivity than EIA methods, which employ absorbance (optical density) measurement.

Chemiluminescent immunoassay (CLIA):

CLIAs utilize a chemiluminescent label. Chemiluminescent molecules produce light when they are excited by chemical energy. These emissions are measured by a light detector.

Counting Immunoassay (CIA):

Involves particle counting technology where latex particles are massed together by antigens or antibodies and are measured by scattered laser light while they are passed through a controlled sheath flow. This method is suitable for antibodies, antigens, haptens, and immune complexes.

Is PCR an immunoassay?

Polymerase chain reaction (PCR) tests are a different type of laboratory method that falls under the category of Molecular Diagnostics and are frequently used to detect HIV's genetic material, called RNA. These tests can be used to screen blood samples and to detect very early infections before antibodies have been developed.

Explore Surmodics IVD’s Molecular Diagnostics Products:

Surmodics IVD’s TRIDIA™ surface coatings optimize DNA, RNA, protein, and cell attachment for molecular diagnostic and immunoassay applications, reducing non-specific background and improving sensitivity. These versatile coatings bind molecules to a variety of surfaces and geometries and may be customized for selectivity using passivating polymers and reactive groups.

For diagnostic devices including point of care assays, Surmodics IVD’s surface coatings improve flow characteristics through membranes and microfluidic channels. Other (non-analyte capture) surfaces from Surmodics IVD increase the wettability.

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