TMB Substrates
TMB Microwell Substrates
The Surmodics™ TMB microwell substrate family outperforms competitors for low background, stability and sensitivity with a variety of kinetic rate substrates. Our one-component formulations provide optimal signal generation and longer shelf lives for ELISA/EIA while streamlining assay manufacturing.
Shop All Products Learn More About TMB Peroxidase SubstratesTMB Membrane/Precipitating Substrates
Surmodics BioFX™® TMB membrane/precipitating substrates outperform competitors for low background, stability and sensitivity.
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BioFX™ One Component HRP Microwell Substrate (TMBW) gives assay developers the best possible tool to maximize shelf life stability and lower backgrounds while setting the industry standard for signal generation of the standard curve.
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Prolonged shelf life. BioFX™ One Component HRP Microwell Substrate (TMBW) maintains 96% activity for over 4 years.
Thirty-two lots spanning 55 months of production were collected and analyzed in a commercial ELISA assay. The color development (Abs @650) was monitored for 30 minutes to determine the kinetic rates across 2 different plates (3 repetitions per substrate on each plate) and the results were normalized to a new lot of TMBW (Age of lot=0 months).
BioFX™ TMB High Definition One Component HRP Microwell Substrate (TMBHD) gives assay developers maximum signal generation and low backgrounds while maintaining shelf life.
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Outstanding Signal Generation For increased sensitivity, BioFX™ TMB substrates generate higher signal per picogram of analyte faster than other TMB substrates.
An IL-6 immunoassay (R&D Systems) was run with TMBHD, TMBW, and other high sensitivity TMB substrates to compare limit of detection and sensitivity. A standard curve was prepared from 100fg/ well to 6.25 fg/well of IL-6. Signal is plotted as the 450nm OD of each well minus the background (zero standard) value. Detection limit was determined by Student’s T test (p < 0.05) at the lowest possible tested analyte concentration.
SENSITIVITY
lot-to-lot consistency
Unparalleled consistency & quality. BioFX™ TMB substrates are manufactured under conditions that provide less than 5% variation among production lots. Surmodics is ISO 13485/9001 certified and is committed to delivering quality products.
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An HRP standard was added to a rabbit anti-HRP antibody coated microplate, incubated and washed. 7 different lots of TMBW were added to designated microwells and absorbance was read every 5 minutes of the kinetic assay.
BOOSTING SENSITIVITY
Surmodics TMB Enhanced HRP Membrane Substrate (ESPM) provides extended dynamic range with reduction of wash out at higher peroxidase concentrations. ESPM demostrates similar sensitivity to most mid-to-high level chemiluminescent subtrates without the costly equipment needed for detection.
Dot blots were prepared with a 1:3 serial dilution of an HRP conjugated antibody starting at 1,000 pg/well, conjugates were incubated and then washed. Membranes were placed in substrate, incubated and read.
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Longer stability
- Up to 4 years of shelf life stability and prolonged room temperature stability
Increased sensitivity
- Increased signal-to-noise ratios and ability to quantitate through the desired assay range
- Multiple formulations with different kinetic rates satisfy the measurable range requirements of any assay
Negligible backgrounds
- Elimination of backgrounds yields lower error through any assay system
Ease of manufacture
- Ready-to-use formulations with reliable performance and long shelf lives
- Manufactured in an ISO 13485/9001 facility for lot-to-lot consistency
TMBC - TMB CONDUCTIVITY
BioFX™ TMB Conductivity One Component HRP Microwell Substrate is a mid-kinetic range substrate for ELISA applications.
TMBHD - TMB High Definition One Component HRP Microwell Substrate
BioFX™ TMB High Definition One Component HRP Microwell Substrate gives assay developers maximal signal generation and low backgrounds while maintaining a long shelf life.
TMBS - TMB Super Sensitive One Component HRP Microwell Substrate
BioFX™ TMB Super Sensitive One Component HRP Microwell Substrate gives assay developers high signal generation and low backgrounds while maintaining a long shelf life.
TMBW - TMB One Component HRP Microwell Substrate
BioFX™ TMB One Component HRP Microwell Substrate gives assay developers the best possible tool to maximize shelf life stability and lower backgrounds while setting the industry standard for signal generation of the standard curve.
TMBX - TMB Extended Range HRP Microwell Substrate
BioFX™ TMB Extended Range HRP Microwell Substrate is a low-kinetic range substrate for ELISA applications.
TMDS - TMB Double Slow One Component HRP Microwell Substrate
BioFX™ TMB Double Slow Kinetic One Component HRP Microwell Substrate is a low-kinetic range substrate for ELISA applications.
TMSK - TMB Slow Kinetic One Component HRP Microwell Substrate
BioFX™ TMB Slow Kinetic One Component HRP Microwell Substrate is a mid-kinetic range substrate for ELISA applications.
TTMB - TMB SUPER SLOW
BioFX™ TMB Super Slow Kinetic One Component HRP Microwell Substrate is a low-kinetic range substrate for ELISA applications.
