ELISA Coating Buffer
When To Use an ELISA Coating Buffer
The primary use for ELISA coating buffers is in immunoassay testing protocols. In a sandwich assay, for example, coating buffers help to immobilize the antibody or antigen coated on the plate. Surmodics™ IVD’s ELISA Coating Buffer (COAT) maximizes specific binding to the target solid phase by providing optimal pH and ionic strength for antigen or antibody binding.
When looking to improve assay sensitivity, stability and specificity, assay developers may utilize diluents, blockers, wash buffers, and other ELISA buffers to improve the assay signal, while reducing background noise. At Surmodics IVD, we excel in creating best-in-class immunoassay reagents for each step of the ELISA procedure.
Frequently Asked Questions
What Is an ELISA Coating Buffer?
Surmodics’ BioFX™ ELISA Plate Coating Solution‐10X Concentrate is a physiological saline solution containing a proprietary blend of monobasic and dibasic phosphates. The solution is ready for dilution to specific application requirements and can be used in most ELISA systems. ELISA Plate Coating Solution‐10X Concentrate maximizes specific binding to the target solid phase by providing optimal pH and ionic strength for antigen or antibody binding.
How Are ELISA Coating Buffers Made?
When assay developers perform preparation protocols for ELISA coating buffers, they must combine the ELISA plate coating solution with distilled water to create a stable solution. Next, they can apply this solution to a standard ELISA plate.
Please reference Surmodics’ recommended protocol for our ELISA Coating Buffer below.
1) To produce a 1X working solution, dilute 1 mL of ELISA Plate Coating Solution‐10X Concentrate in 9 mL of reagent quality water.
2) Allow working solution to equilibrate to room temperature (25°C) prior to use.
3) Dilute antigen or antibody to desired concentration in 1X solution and treat the target surface by covering completely with solution.
o For best results, incubate coated plate overnight at 4°C.
What Buffer Is Typically Used in the ELISA Test?
Several buffers can be used in an ELISA application. Here at Surmodics™ IVD, we provide assay developers the ELISA buffers they need to build robust, accurate and reproducible ELISA’s. Our extensive portfolio of ELISA buffers helps to increase the sensitivity, specificity and stability of ELISA’s. In addition, each of our ELISA buffers were designed to help tackle difficult technical issues such as non-specific binding, matrix interferences and more; in turn, our ELISA buffers help assay developers in the continued optimization of their ELISA technology.
Assay developers utilize various ELISA buffers to reduce non-specific binding to unwanted proteins during the incubation of diagnostic samples. ELISA buffers such as, sample diluents help reduce background noise and the risk for false positives.
Visit the link below to learn more about our ELISA buffers.
More than a Supplier
At Surmodics, we remain dedicated to improving the detection and treatment of disease. Our immunoassay reagents offer assay developers the gold standard in product performance and offer assay developers a complete set of tools for building consistent and reliable immunoassays.
Our immunoassay reagents are designed to increase the sensitivity, specificity and stability of immunoassays, including ELISA’s, point-of-care devices and more diagnostic applications. These immunoassay reagents are critical for the accuracy and reliability of immunoassays.
Surmodics IVD is staffed by scientists with expertise in chemistry, biochemistry, molecular biology, and cell biology with vast combined experience in IVD product development. As an extension of your development team, they’re eager to dig deep into any challenge you face, regardless of the source.
To learn more about our products and mission at Surmodics, browse our industry-leading solutions, check out our blog, or contact us directly to connect with one of our technical team members today!
ELISA Coating Buffer 10x Concentrate
The solution is ready for dilution to specific application requirements and can be used in most ELISA systems. ELISA Plate Coating Solution-10X Concentrate maximizes specific binding to the target solid phase by providing optimal pH and ionic strength for antigen or antibody binding.