ELISA Blocking Reagents

Surmodics™ IVD has a wide selection of reliable, ready-to-use ELISA blocking reagents for assay manufacturing companies and laboratory professionals looking to build sensitive, reproducible immunoassays. We offer several blocking reagents for immunoassays, including point-of-care applications, ELISA kits and more. Each of Surmodics IVD’s ELISA blocking reagents offer assay developers the gold standard in product performance and quality. Our ISO 13485:2016 and 9001:2015 certifications allow us to provide a level of unmatched consistency and quality.

Our ELISA blocking reagents include a line of dried protein stabilizers and blockers, sample or assay diluents and western blot blocking buffers. Our ELISA blocking reagents are designed to increase the sensitivity and stability of immunoassays, including ELISA applications while blocking interferences that impact the accuracy and reliability of immunoassays. Furthermore, our ELISA blocking reagents help to reduce cross-reactivity and interferences from Human Anti-Mouse Antibodies (HAMA) and Rheumatoid Factor (RF) as well as non-specific binding.

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Characteristics of Ideal ELISA Blocking Reagents

As an assay developer, it is critical to find an ELISA blocking reagent that offers optimal blocking for your specific immunoassay needs.

In your search for ELISA blocking reagents, it can be helpful to understand the key characteristics that ELISA blocking reagents offer.

  1. Reduces the risk for non-specific binding from the sample matrix and other assay components.
  2. Offers extended shelf life of dried proteins on various surfaces, including polystyrene plates, latex beads, magnetics particles and nitrocellulose membranes.
  3. Provides increased signal-to-noise ratios across the assay range.
  4. Prevents denaturation and act as a stabilizer for solid phase assay reactants.
  5. Reduces cross-reactivity with any other components of the assay.
  6. Should not have any kind of activity that is enzymatic, since this could possibly lead to the degradation of the reactants or interfere with the generation of a signal from the substrate.
  7. Offers consistent performance across multiple assay formats and should also ensure lot-to-lot consistency.

The main characteristic that determines if a blocking buffer is effective is that it should reduce the background signal and improve the ratio of signal-to-noise, thus improving the overall sensitivity of an assay. Surmodics IVD’s ELISA blocking reagents help to increase the signal-to-noise ratios and in turn, enhance the accuracy and reliability of immunoassays.

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Specific Families of Blocking Reagents

Dried Protein Stabilizers & Blockers:

Surmodics IVD’s StabilCoat™, StabilGuard™ and StabilBlock™ dried protein stabilizers and blockers preserve the conformation and activity of dried proteins coated on a wide range of surfaces, keeping antibodies and antigens at peak performance for long durations. At the same time, the blocking mechanisms in these reagents reduce non-specific binding of interfering proteins to maximize assay sensitivity. These ELISA blocking reagents are the industry gold standard for stability and blocking efficacy. The result is immediate improvement of assay performance in a one-step process for streamlined manufacturing.

Assay developers need numerous blocking mechanisms while selecting the antibody titration during assay optimization. Our dried protein stabilizers and blockers provide alternative blocking mechanisms to achieve the best signal-to-noise ratios for each assay. With the introduction of StabilBlock, Surmodics IVD now offers the best available reagent for reducing non-specific binding to boost immunoassay signal-to-noise ratios.

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Assay/Sample Diluents:

Surmodics IVD’s ELISA blocking reagents also include sample or assay diluents that help to reduce matrix interferences in the assay. Surmodics Assay Diluent and MatrixGuard™ Diluent are the gold standard for reducing false positives in your assay. Both formulations provide two options to use when different methods are needed to block matrix interferences, while maintaining the clinical utility of the assay.

For IVD kit manufacturers requiring a strong, consistent blocking diluent across a variety of assays, MatrixGuard Diluent provides unsurpassed blocking whereby matrix interferences are effectively blocked while the intended assay signal is maintained. Unlike other diluents that either are marginally effective at blocking matrix interferences or alternatively block out true assay signal, MatrixGuard Diluent achieves the goal of maximum blockade of matrix interferences while simultaneously allowing signal to be maintained.

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Western Blot Blocking Buffers:

In addition, we also offer blocking reagents for western blot applications including a variety of milk, casein and protein-free blockers. Our StabilBlot™ blocking buffer family enhances sensitivity and minimizes background in blotting applications.

Western blotting, also known as immunoblotting, is a method used to detect a target protein. Western blotting combines the protein separation capabilities of PAGE (polyacrylamide gel electrophoresis) and immunochemistry to detect these target proteins from a complex matrix such as cell or tissue lysate. Membranes used in western blotting are porous and should be blocked to avoid nonspecific binding and background.

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Choosing the Right Blocking Reagent

There is a wide variety of blocking buffer solutions that can be used for a wide range of applications and each has their own advantages and disadvantages. Obtaining information on which blocking buffer is best to be used in various applications is a critical component in accurate testing results. When selecting a blocking buffer, the most important characteristic to look for is a blocking buffer that will yield the optimal signal-to-noise ratio. It is also important to choose a blocking buffer that does not contain any substances that might interfere with the assay.

Here is more information on how to obtain optimal results from blocking experiments:

  • Use various blocking reagents for experiments and monitor the signal strength and background in each.
  • Select a blocking buffer that will yield the highest signal-to-noise ratio
  • Make sure that there are not any substances that are within the blocking buffer that might interfere with the assay’s performance.