Tissue Transglutaminase (tTG; expressed in E.coli)

Tissue transglutaminase (tTG; syn. TGase-2) is an ubiquitously expressed enzyme, which exerts protein deamidation and transamidation activity leading to the creation of neo-epitopes and the cross-linking of proteins, respectively. DIARECT™s tTG has been sequence modified to eliminate this cross-linking activity and to prevent covalent aggregates of tTG with host cell proteins. DIARECT™ antigens are for further manufacturing or research use only.

Origin
Recombinant. Expressed in E. coli bacterial cells.
Biochemical Test
SDS-PAGE (purity > 95%); Western blot with i: anti-tTG autoantibody positive sera; ii: monoclonal anti-hexa-His-tag antibody; immunodot with anti-tTG autoantibody positive sera.
Calculated Molecular Weight
78,018 Dalton
Calculated Isoelectric Point
pH 5.3
Coating Concentration
0.6-1.4 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups. Remark: very diverse coating protocols exist in the literature; DIARECT recommends extensive testing in assay development.  

A144


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Option Quantity Unit Purchase
TISSUE TRANSGLUTAMINASE
A14400
0.1 mg
$160.00/EA
In Stock
TISSUE TRANSGLUTAMINASE
A14401
1.0 mg
$1,620.00/EA
In Stock

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