SmD3

Origin

Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells. Expression conditions allow for introduction of symmetrical arginine dimethylation, thereby creating the main SmD3 autoepitope.

Biochemical Test

SDS-PAGE (purity > 80%); Western  blot with anti-Sm autoantibody-positive sera.

Calculated Molecular Weight

15 kDa 

Calculated Isoelectric Point

pH 10.33

Coating Concentration

0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

A120