SmD

In eukaryotic cells, the Sm-proteins B/B’, D1, D2, D3, E, F, and G form a heteroheptameric protein core shared between all uracil-rich small nuclear ribonucleoprotein (U-snRNP) complexes. SmD comprises equal masses of SmD1, SmD2, and SmD3. In addition, both SmD1 and SmD3 contain symmetrically dimethylated arginine residues, which represent a major epitope. DIARECT™ antigens are for further manufacturing or research use only.

Origin
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells under conditions promoting symmetrical dimethylation of arginine residues.
Biochemical Test
SDS-PAGE (purity > 80%); Western blot with i: anti-Sm autoantibody-positive sera; ii: monoclonal anti-hexa-His-tag antibody.
Calculated Molecular Weight
SmD1 14,306 Dalton SmD2 14,773 Dalton SmD3 14,739 Dalton
Calculated Isoelectric Point
SmD1 pH 11.56 SmD2 pH 9.92 SmD3 pH 10.33
Coating Concentration
0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

A117


Filter
Option Quantity Unit Purchase
SmD
A11700
0.1 mg
$130.00/EA
In Stock
SmD
A11701
1.0 mg
$1,505.00/EA
In Stock

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