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SAE1/SAE2

SAE1 and SAE2 form heterodimers that are involved in the posttranslational modification of proteins (sumoylation). This process regulates protein structure as well as intracellular localization of the target. SAE1/2 serves as a diagnostic marker for dermatomyositis (DM) as autoantibodies against the two proteins have been isolated from patients. DIARECT™ antigens are for further manufacturing or research use only.

Origin
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells.
Biochemical Test
SDS-PAGE (purity > 80%); Western-Blot with i: anti-SAE1/SAE2 autoantibody positive sample; ii: polyclonal anti-SAE1 and anti-SAE2 antibodies
Calculated Molecular Weight
SAE1subunit: 39 kDa SAE2 subunit: 73 kDa
Coating Concentration
0.3-0.8 µg/mL (depending on the type of ELISA plate and coating buffer) Suitable for labeling of functional groups

A316


Option Unit Purchase
SAE1/SAE2
A31600
$150.00/EA
In Stock
SAE1/SAE2
A31601
$1,710.00/EA
In Stock
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