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Proliferating Cell Nuclear Antigen (PCNA)

Proliferating cell nuclear antigen (PCNA; syn. Cyclin) is a co-factor of DNA polymerase delta, forms homotrimers around double stranded DNA, and is involved in DNA replication and repair. Since its expression rate correlates with the rate of DNA replication, PCNA is a proliferation marker in basic research. DIARECT™ antigens are for further manufacturing or research use only.

Origin
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells.
Biochemical Test
SDS-PAGE (purity> 90%); Western blot with i: anti-PCNA autoantibody­positive sample; ii: monoclonal anti-­His-tag antibody.
Calculated Molecular Weight
30 kDa
Calculated Isoelectric Point
pH 4.8
Coating Concentration
0.5-1.1 µg/ml (depending on the type of ELISA plate and coating buffer; CAUTION: these values are not based on exhaustive testing and are only guidelines for customer's development work). Suitable for labeling of functional groups.

A154


Filter
Option Quantity Unit Purchase
PROIFERATING CELL NUCLEAR
A15400
 50 µg
$150.00/EA
In Stock
PROIFERATING CELL NUCLEAR
A15401
 1.0 mg
$1,710.00/EA
In Stock
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