M2

Mitochondrial 2-oxo acid dehydrogenase multienzyme complexes play a pivotal role in the regulation of the cellular redox state. M2 is the synonym for a cellular antigen comprising the E2-subunits of distinct dehydrogenase complexes, namely PDC-E2, OGDC-E2, and BCOADC-E2. DIARECT™ antigens are for further manufacturing or research use only.

Origin
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells.
Biochemical Test
SDS-PAGE (purity > 75%); Western blot with i: anti-M2 autoantibody-positive sera; ii: monoclonal anti-hexa-His-tag antibody.
Calculated Molecular Weight
PDC-E2 60,630 Dalton OGDC-E2 42,301 Dalton BCOADC-E2 47,321 Dalton
Calculated Isoelectric Point
PDC-E2 pH 5.8 OGDC-E2 pH 6.3 BCOADC-E2 pH 6.5
Coating Concentration
0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.  

A180


Filter
Option Quantity Unit Purchase
M2
A18000
50 μg
$140.00/EA
In Stock
M2
A18001
1.0 mg
$1,595.00/EA
In Stock

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