Intrinsic Factor (IF)

Cobalamin/vitamin B12 is essential for the formation of blood cells and DNA synthesis, and needs to be taken up with the diet. Intrinsic factor, which is secreted by the stomach, binds to vitamin B12 in the small intestine to allow its endocytosis in the ileum. DIARECT™ antigens are for further manufacturing or research use only.

Origin
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells. Purification of recombinant IF includes steps for removal of bound vitamin B12.
Biochemical Test
SDS-PAGE (purity > 80%); Western blot with anti-hexa-His-tag antibody.
Calculated Molecular Weight
44,228 Dalton (protein component excluding glycosylation; observed molecular weight in SDS-PAGE is approx. 55 kDa).
Calculated Isoelectric Point
pH 6.3
Coating Concentration
0.25-0.65 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.  

A167


Filter
Option Quantity Unit Purchase
INTRINSIC FACTOR (IF)
A16700
50 μg
$140.00/EA
In Stock
INTRINSIC FACTOR (IF)
A16701
1.0 mg
$1,595.00/EA
In Stock

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