Gliadin (recombinant; deamidated)

Gliadin describes a class storage proteins found in wheat grains. Based on its solubility, α-,β-,γ-, and ω-gliadin can be distinguished. Gliadin is a common substrate of transglutminase, which generates neo-epitopes by deamidation of glutamine side chains. The sequence of DIARECT™s Gliadin fragment has been design to contain these neo-epitopes. DIARECT™ antigens are for further manufacturing or research use only.

Origin
Recombinant. Expressed in E. coli bacterial cells.
Biochemical Test
SDS-PAGE (purity> 80%); Western blot with anti-Gliadin autoantibody-positive sample. .
Calculated Molecular Weight
19 kDa (high proline content and the low pl are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 50 kDa on standard SDS-PAGE).
Calculated Isoelectric Point
pH 4.6
Coating Concentration
0.15-0.5 µg/ml (depending on the type of ELISA plate and coating buffer).   

A195


Filter
Option Quantity Unit Purchase
GLIADIN
A19500
50 μg
$140.00/EA
In Stock
GLIADIN
A19501
1.0 mg
$1,595.00/EA
In Stock

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