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DNA Topoisomerase I (Scl-70; full length)

DNA Topoisomerase I is an enzyme that adjusts the supercoiling stage of DNA to resolve torsional stress occurring during DNA transcription and replication. Proteolytical processing matures the 100 kDa precursor into the 70 kDa form. Scl-70 full length corresponds to the 100 kDa precursor. DIARECT™ antigens are for further manufacturing or research use only.

Origin
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells.
Biochemical Test
SDS-PAGE (purity> 80%); Western blot with i: anti-Scl-70 autoantibody­positive sample; ii: monoclonal anti­His-tag antibody.
Calculated Molecular Weight
92 kDa
Calculated Isoelectric Point
pH 9.3
Coating Concentration
0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Filter
Option Quantity Unit Purchase
DNA TOPOISOMERASE l
A12400
 50 µg
$150.00/EA
In Stock
DNA TOPOISOMERASE l
A12401
 1.0 mg
$1,710.00/EA
In Stock
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